Evaluation of in Vitro Anticancer Activity of Hydroalcoholic Extract of Tabernaemontana Divaricata

Tabernaemontana divaricata a native of India and many other tropical regions is a common garden plant that has been used traditionally for treatment of a number of diseases. In the current study the hydroalcoholic extract of the flowers of the plant have been tested for anticancer activity. The extract was prepared by soxhlet extraction method, petroleum ether and hydroalcohol were the solvents used. The in-vitro anticancer studies were performed against human cancer cell line (HeLa) and MTT assay was used to analyze the cell growth inhibition. The results showed that the hydroalcoholic extract of flowers of T.divaricata possessed a moderate amount of anticancer activity and the IC50 value was greater than100 μg/ml.